E-Abstract

JACC

Lots of interesting abstracts and cases were submitted for TCTAP 2023. Below are the accepted ones after a thorough review by our official reviewers. Don¡¯t miss the opportunity to expand your knowledge and interact with authors as well as virtual participants by sharing your opinion in the comment section!

TCTAP A-071

Comparison of Perindopril vs. Losartan With Regard to Pro-Coagulation Factors in Adipocytes Exposed to Sars-Cov-2 Spike Protein

By Hanestya Oky Hermawan, Primasitha Maharany Harsoyo, Makhyan Jibril Al-farabi, I Gde Rurus Suryawan, Meity Ardiana

Presenter

Hanestya Oky Hermawan

Authors

Hanestya Oky Hermawan1, Primasitha Maharany Harsoyo1, Makhyan Jibril Al-farabi2, I Gde Rurus Suryawan3, Meity Ardiana2

Affiliation

General Regional Academic Hospital Dr. Soetomo, Universitas Airlangga, Indonesia1, Airlangga University, Indonesia2, Dr. Soetomo Academic General Hospital, Indonesia3
View Study Report
TCTAP A-071
Basic Science, Animal Models and Preclinical Studies

Comparison of Perindopril vs. Losartan With Regard to Pro-Coagulation Factors in Adipocytes Exposed to Sars-Cov-2 Spike Protein

Hanestya Oky Hermawan1, Primasitha Maharany Harsoyo1, Makhyan Jibril Al-farabi2, I Gde Rurus Suryawan3, Meity Ardiana2

General Regional Academic Hospital Dr. Soetomo, Universitas Airlangga, Indonesia1, Airlangga University, Indonesia2, Dr. Soetomo Academic General Hospital, Indonesia3

Background

Obesity is a strong risk factor for more severe Covid-19 infection as adipocytes play an important role in intermediating the spreading, replication, and release of SARS-COV-2. An increase in pro-coagulation factors (tissue factor (TF) and plasminogen activator inhibitor-1 (PAI-1)) was observed in Covid-19 patients with moderate to severe symptoms and is reported to be associated with Angiotensin-converting enzyme 2 (ACE2) overexpression. Cardiovascular medications affecting ACE2, such as Perindopril and Losartan, are hypothesized to have an effect on Covid-19 infection-related coagulopathy. This study aims to identify and compare the effect of perindopril and losartan on TF and PAI-1 levels in adipocytes exposed to SARS-COV-2 spike protein. 

Methods

Adipocytes were isolated enzymatically from adipose tissue obtained from an obese male donor. Adipocytes were then exposed to SARS-COV-2 S1 spike protein for 24 hours. After exposure, perindopril and losartan were added to the culture medium. ACE2, TF, and PAI-1expression were measured 2 hours later using ELISA.

Results

SARS-CoV-2 spike protein exposure increased ACE2, TF, and PAI-1 expression. Perindopril addition discernible reduced the tissue factor (TF) expression (4.843 ¡¾ 0.396) compared to a positive control (6.857 ¡¾ 0.228) (p=0.005) but not losartan(5.624 ¡¾ 0.606) (p=0.111). Perindopril was also able to lower PAI-1 expressions (3.484 ¡¾ 0.252) compared to a positive control (4.865 ¡¾ 0.115) (p=0.001), but the losartan did so more effectively (2.633 ¡¾ 0.269) (p=0.000).

Conclusion

Losartan and perindopril both have the ability to lower pro-coagulation factors, proving the value of ACEIs/ARBs in preventing thrombotic complications in Covid-19 patients.