Improved Effect of Bone Marrow Stem Cells Myocardial Transplantation By Ultrasound Mediated Microbubble Destruction
Zhongda Hospital, Nanjing, China
J.Y. Tong, Y. Feng, G.S. Ma, Z. Xu, Y.M. Su, Y.P. Hu, J.D. Ding
Background: To investigate the effects of ultrasound-mediated microbubble destruction on bone marrow stem cells(BMSCs) myocardial transplanting in the animal level. this method will provide a new thinking about the efficiency of BMSCs myocardial transplantation for myocardial infarction patients.
Methods: 12 pigs are random divided into control group (group A, n=4)and experimental group(group B, n=8) .according to killed time ,the group B is divided into group B1( after transplanting 0 hours, n=4) and group B2(24 hours, n=4).12 pigs are taken suction the bone marrow, dysjunction, BMSCs purely , cultivanted, and labeled with superparamagnetic iron oxide nanoparticles （SPIO）respectively , 12 pigs are subjected to closed-chest experimental myocardial infarction (MI) model. Labeled BMSCs（1~5x106）are infused intracoronarily under x-ray fluoroscopy after 14 days that acute myocardial infarction(AMI) mode are made. Group A is pure infused BMSCs into myocardium through left anterior descending branch, group B is infused microbubble (Sono Vue) at first, and then infused BMSC into myocardium through left anterior descending branch, at the same time the region of MI is applied ultrasound (1 MHz,2 W/ cm2, continue to radiate 90 second). Magnetic resonance imaging( MRI) are obtained on a 1.5T MR scanner to demonstrate the location of the BMSCs in living body pigs after operation 0,24 hours respectively, and compared with histology.
Results: 1)MRI: The zone of SPIO labeled BMSCs accumulation shows vague darkening on T2* weighted MRI in both group A and group B. 2) Prussian blue staining: Film preparation of myocardials digests and histological analyses showed experimental group have most Prussian blue staining positive cells than control group have. ( P<0.01).3）transmission electron microscope: The myocardial ultrastructure in two groups has no difference, but the gap of blood vessel endothelium widens in group B1, group B2 has not this variety.
Conclusion: This study demonstrates that ultrasound destruction of microbubbles enhances BMSCs myocardial transplantation efficiency, the mechanism may have something to do with ultrasound destruction of microbubbles causing of the gap of blood vessel endothelium reversibility widen, this method will provide a new thinking about increasing BMSCs myocardial transplantation efficiency.
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